REVERSED-PHASE CHROMATOGRAPHY OF PROTEINS AND PEPTIDES ON COMPRESSED CONTINUOUS BEDS

The polymer beds described are synthesized in aqueous solution directl y in the column or batchwise in the form of large clusters of small pa rticles. The conventional, expensive step involving prepreparation of beads in an organic solvent is thus omitted. Beds were synthesized fro m piperazine diacrylamide, methacrylamide and allyl glycidyl ether. Th e epoxy-activated beds thus obtained were used for covalent attachment of either nonpolar ligands (e.g. octadecanol) or polar OH-rich substa nces (e.g. dextran). The non-polar beds were used for reversed-phase c hromatography, as were polar ones following coupling with 1,2-epoxyoct adecane. Coating with OH-rich substances serves two purposes: (1) the matrix becomes hydrophilic, decreasing nonspecific interactions (modif iers can be excluded) and hence increases resolution and (II) many - O H groups are available (e.g. for coupling to epoxides), a prerequisite for high ligand density. Resolution of proteins was high even at high flow rates. Depending on the method used for the synthesis of the bed , resolution of proteins either increased with an increase in flow rat e or decreased slightly. Choice of the correct temperature was very im portant for high resolution of CNBr-digested peptides.