RETENTION OF SOME GLUCOSINOLATES IN ANION-EXCHANGE CHROMATOGRAPHY .1.QUALITATIVE STUDY ON A SILICA-TRIALKYLAMMONIUM EXCHANGER

The retention and separation of glucosinolates, as organic anions, wer e studied on a silica-based strong anion exchanger under isocratic elu tion conditions. All glucosinolates carry the same functional ionic gr oup (-OSO3-), however they do not have the same retention in anion exc hange chromatography. The plots of capacity factors of organic anions versus the reciprocal of eluent ion concentration show good linearity. From the slope and y-intercept data the major retention mechanisms ar e interpreted as ion exchange, and reversed-phase interactions. The ef fects of nature and concentration of the eluent ion and the influence of organic modifier addition to the aqueous buffered mobile phase are also investigated. Direct and indirect UV detection were used. Our res ults open the way for the development of new systems for intact glucos inolate analysis which are easier to use than the present ion-pairing chromatographic method.