ASSOCIATION OF NUCLEOSIDES AND THEIR 5'-MONOPHOSPHATES WITH A TRYPTOPHAN CONTAINING TRIPEPTIDE, TRP-LEU-GLU - THE SOURCE OF AN OVERESTIMATION BY FLUORESCENCE SPECTROSCOPY

Authors
WIECZOREK Z STEPINSKI J DARZYNKIEWICZ E LONNBERG H
Citation
Z. Wieczorek et al., ASSOCIATION OF NUCLEOSIDES AND THEIR 5'-MONOPHOSPHATES WITH A TRYPTOPHAN CONTAINING TRIPEPTIDE, TRP-LEU-GLU - THE SOURCE OF AN OVERESTIMATION BY FLUORESCENCE SPECTROSCOPY, Biophysical chemistry, 47(3), 1993, pp. 233-240
Citations number
23
Categorie Soggetti
Biophysics,Biology,"Chemistry Physical
Journal title
Biophysical chemistry → ACNP
ISSN journal
03014622
Volume
47
Issue
3
Year of publication
1993
Pages
233 - 240
Database
ISI
SICI code
0301-4622(1993)47:3<233:AONAT5>2.0.ZU;2-P
Abstract
Association of 7-methylguanosine 5'-monophosphate with a tryptophan co ntaining tripeptide, Trp-Leu-Glu, has been studied by fluorescence tit ration using two different geometries of detection, viz. right angle a nd front surface geometry. The applicability of these two techniques t o determine the stability constant of the nucleotide-peptide adduct is discussed. Evidence is presented that fluorescence titration based on right angle detection may lead to considerable overestimation of the strength of interaction.