GAMETIC ASSOCIATION OF HSP70-1 PROMOTER REGION ALLELES AND THEIR INCLUSION IN EXTENDED HLA HAPLOTYPES

Gametic associations of a three-allele polymorphism of the HSP70-1 pro moter region were analyzed in a random North Italian population, in 69 HLA homozygous cell lines and in 29 families in Boston, all typed for HLA class 1, class 11 and complement alleles. Significant phenotypic associations were detected in the random population between HSP70-1 al leles and several HLA markers carried by extended haplotypes. The incl usion of HSP70-1 alleles in extended haplotypes, suggested by populati on analysis, was confirmed in genotyped cells, including 10W HLA homoz ygous cell lines and families, selected for the presence of the whole set of alleles reported for conserved extended haplotypes. Every teste d extended haplotype was exclusively associated with a given HSP70-1 a llele, except those carrying DR1. HSP70-1 C was included in both [HLA- B8, SC01, DR3] and [HLA-B18, FIC30, DR3] extended haplotypes, accounti ng for the previously observed strong association with DR3. In additio n the same allele was found on the [HLA-B13, SC31, DR7], on the [HLA-B 62, SB42, DR4] and on the [HLA-B60, SC02, DR13] extended haplotypes. T he HSP70-1 A allele was carried by all DR4+ extended haplotypes except the one above cited. HSP70-1 B correlated with DR10, DQB10501 and BF E Thus the HSP70-1 promoter alleles provide new precisely located mar kers of extended haplotypes.