INHIBITION OF THE FRIEND RETROVIRUS BY ANTISENSE OLIGONUCLEOTIDES ENCAPSULATED IN LIPOSOMES - MECHANISM OF ACTION

Citation
C. Ropert et al., INHIBITION OF THE FRIEND RETROVIRUS BY ANTISENSE OLIGONUCLEOTIDES ENCAPSULATED IN LIPOSOMES - MECHANISM OF ACTION, Pharmaceutical research, 10(10), 1993, pp. 1427-1433
Citations number
22
Categorie Soggetti
Pharmacology & Pharmacy",Chemistry
Journal title
ISSN journal
07248741
Volume
10
Issue
10
Year of publication
1993
Pages
1427 - 1433
Database
ISI
SICI code
0724-8741(1993)10:10<1427:IOTFRB>2.0.ZU;2-N
Abstract
Proliferation of the Friend retrovirus was specifically inhibited by t he env mRNA complementary oligonucleotide encapsulated in pH-sensitive liposomes. This observation was made using the focus immunoassay (FIA ) and the reverse transcriptase test. The key finding of the present s tudy was the dramatic impact on liposome penetration. For chronic or d e novo infection, the point at which the penetration of liposomes bega n corresponded to the time needed for the virus to leave the cell. In the absence of the virus, liposomes remained adsorbed onto the cell su rface without any internalization. Regardless of the mechanism involve d, the fact that a retroviral infection stimulates the cellular uptake of oligonucleotide liposomes widens the spectrum of strategies for sp ecific antiviral action.