RAPID AUXIN-INDUCED ENHANCEMENT OF PROTEIN-BIOSYNTHESIS IN RYE COLEOPTILES

The effect of auxin (indole-3-acetic acid, IAA) on growth and incorpor ation of H-3-leucine into cytoplasmic and cell-wall bound proteins of abraded coleoptile sections from rye seedlings (Secale cereale L.) was investigated. In the absence of IAA (water control) a strong incorpor ation of tracer into both protein fractions was observed. Auxin rapidl y increased the incorporation of H-3-leucine into both cytoplasmic and cell-wall fractions of the coleoptiles in close temporal corresponden ce to the growth response of the sections. The uptake of tracer was un affected by the hormone. However, auxin did not alter the pattern of p rotein synthesis as measured by H-3-leucine labelling, i.e. we were un able to detect a specific polypeptide that is synthesized de novo in r esponse to IAA.