FINE REGULATION OF CI857-CONTROLLED GENE-EXPRESSION IN CONTINUOUS-CULTURE OF RECOMBINANT ESCHERICHIA-COLI BY TEMPERATURE

Authors
VILLAVERDE A BENITO A VIAPLANA E CUBARSI R
Citation
A. Villaverde et al., FINE REGULATION OF CI857-CONTROLLED GENE-EXPRESSION IN CONTINUOUS-CULTURE OF RECOMBINANT ESCHERICHIA-COLI BY TEMPERATURE, Applied and environmental microbiology, 59(10), 1993, pp. 3485-3487
Citations number
20
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
59
Issue
10
Year of publication
1993
Pages
3485 - 3487
Database
ISI
SICI code
0099-2240(1993)59:10<3485:FROCGI>2.0.ZU;2-1
Abstract
The expression at different temperatures of the lacZ gene, which is co ntrolled by the lambda p(L) and P(R) tandem promoters and the cI857 te mperature-sensitive repressor, was studied in Escherichia coli continu ous cultures. At temperatures between 30 and 42-degrees-C, beta-galact osidase activity behaved according to an exponential equation. By indu cing a culture at a temperature within this range, predefined, nearly constant submaximal levels of gene expression and recombinant product yield can be obtained.