EFFECTS OF GLUCOSE, GROWTH TEMPERATURE, AND PH ON LISTERIOLYSIN-O PRODUCTION IN LISTERIA-MONOCYTOGENES

Citation
Ar. Datta et Mh. Kothary, EFFECTS OF GLUCOSE, GROWTH TEMPERATURE, AND PH ON LISTERIOLYSIN-O PRODUCTION IN LISTERIA-MONOCYTOGENES, Applied and environmental microbiology, 59(10), 1993, pp. 3495-3497
Citations number
14
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
00992240
Volume
59
Issue
10
Year of publication
1993
Pages
3495 - 3497
Database
ISI
SICI code
0099-2240(1993)59:10<3495:EOGGTA>2.0.ZU;2-F
Abstract
Expression of listeriolysin O of Listeria monocytogenes as a function of different growth conditions was studied by performing a direct hemo lysin assay, immunoblotting experiments, and an enzyme-linked immunoso rbent assay. Expression of listeriolysin O was reduced at a lower grow th temperatures (26-degrees-C) and at higher glucose concentrations (g reater-than-or-equal-to 0.3%) in the growth media. The effect of gluco se appeared to be due to a change in the pH of the growth media.