INDUCTION AND REGENERATION OF AUTOPLASTS FROM CLOSTRIDIUM-THERMOHYDROSULFURICUM JW102 AND THERMOANAEROBACTER-ETHANOLICUS JW200

Authors
PETERANDERL R CANGANELLA F HOLZENBURG A WIEGEL J
Citation
R. Peteranderl et al., INDUCTION AND REGENERATION OF AUTOPLASTS FROM CLOSTRIDIUM-THERMOHYDROSULFURICUM JW102 AND THERMOANAEROBACTER-ETHANOLICUS JW200, Applied and environmental microbiology, 59(10), 1993, pp. 3498-3501
Citations number
20
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
59
Issue
10
Year of publication
1993
Pages
3498 - 3501
Database
ISI
SICI code
0099-2240(1993)59:10<3498:IAROAF>2.0.ZU;2-J
Abstract
Autolysis was induced to form stable, cell wall-free cells of Clostrid ium thermohydrosulfuricum JW102 and Thermoanaerobacter ethanolicus JW2 00, using a complex medium containing glycine (0.4% wt/vol) and/or suc rose or glycerol (10% wt/vol) at an optimum temperature of 64-degrees- C. Autoplasts of both bacteria were grown as L-phase colonies on solid medium; more than 50% of these colonies regenerated to the walled for m during prolonged incubation. The removal of the cell wall was confir med by electron microscopy.