NEW PHOSPHITYLATING REAGENT IN THE NUCLEOTIDE CHEMISTRY CONTAINING 2 4-NITROPHENOXY LEAVING GROUPS - REMARKABLY FAST AND CLEAN PHOSPHITYLATIONS ACTIVATED BY DBU LEADING TO THIO-OLIGONUCLEOTIDES AND SELENO-OLIGONUCLEOTIDES

Authors
HELINSKI J DABKOWSKI W MICHALSKI J
Citation
J. Helinski et al., NEW PHOSPHITYLATING REAGENT IN THE NUCLEOTIDE CHEMISTRY CONTAINING 2 4-NITROPHENOXY LEAVING GROUPS - REMARKABLY FAST AND CLEAN PHOSPHITYLATIONS ACTIVATED BY DBU LEADING TO THIO-OLIGONUCLEOTIDES AND SELENO-OLIGONUCLEOTIDES, Tetrahedron letters, 34(40), 1993, pp. 6451-6454
Citations number
7
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron letters → ACNP
ISSN journal
00404039
Volume
34
Issue
40
Year of publication
1993
Pages
6451 - 6454
Database
ISI
SICI code
0040-4039(1993)34:40<6451:NPRITN>2.0.ZU;2-L
Abstract
A new synthetic approach to modified oligonucleotides based on N,N'-di isopropyl-di- (4-nitrophenyl)phosphoroamidite 1 is described. The proc edure involves displacement of either 4-nitrophenoxy or diisopropylami no ligands. The former proceeds very fast in the presence of DBU, the latter requires activation by tetrasole. This method allows rapid and almost quantitative construction of modified nucleotides derived from thio- and selenophosphorus acids.