WILL MOLECULAR-BIOLOGY CONTRIBUTE TO REFINE PROGNOSIS AND TO SELECT TREATMENT

Authors
ZACCARIA A MARTINELLI G BUZZI M FARABEGOLI P SAGLIO G GUERRASIO A BERNABEI PA SANTINI V PELLICCI PG MENCARELLI A COCO FL DIVERIO D RAMBALDI A SANTORO A TURA S RUSSO D ZUFFA E FANIN R PATRIARCA F FIACCHINI M BACCARANI M TESTONI N ZAMAGNI MD ZACCARIA A MONTEFUSCO E ALIMENA G MELONI G MANDELLI F DAMIANI D MICHIELI M CRISCUOLO D FOWST C HOLDENER EE SPECCHIA G LISO V MORRA E BERNASCONI C DEMILIO A BATTISTA R DITUCCI A BROCCIA G MAIOLINO I CARONIA F LUCIANO L ROTOLI B LEONI P DANIELI G BODENIZZA CA CAROTENUTO M ROTONDO SG NOSARI A DECATALDO F MONTUORO A DELAURENZI A CAMILLO S LIBERATI AM GRIGNANI F TABILIO A MARTELLI M RAMBALDI A BARBUI T LEONI F CIOLLI S RONCA F NOBILE F PAOLINO F RESEGOTTI L PAPINESCHI F SPREMOLLA G LANDOLFI R LEONE G VOLPE E MANGONI L RIZZOLI V CAPUCCI A IZZI T SCAPOLI GL CASTOLDI GL GENTILINI I COSER P GALLO E PILERI A CANTONETTI M PAPA G DINI D MORANDI S BIANCHINI E PINOTTI G VENCO A ZAGONEL V PINTO A CAPALDI A GIOVANNELLI E PIZZUTI M RICCIUTI F AMBROSETTI A CAJOZZO A LOMBARDO M TORLONTANO G DELFINI C LUCARELLI G GIRINO M ASCARI E RISSO M DAMASIO E MARTINO S PARDINI S LONGINOTTI M MIRAGLIA E DEBIASE R NARDELLI S GALIENI P DISPENSA E ABBADESSA A BRUZZESE L PROSSOMARITI L CIMINO R DEROSA C GABBAS A FRANCESCO S GALLAMINI A DIFRANCESCO A QUAGLINO D MUSOLINO C SQUADRITO G AVANZINI P GOBBI F NUOVA ASM EMILIA R SAGLIO G AGLIETTA M CAMASCHELLA C MAZZA MU GUGLIELMO P CACCIOLA E MONACO M
Citation
A. Zaccaria et al., WILL MOLECULAR-BIOLOGY CONTRIBUTE TO REFINE PROGNOSIS AND TO SELECT TREATMENT, Leukemia & lymphoma, 11, 1993, pp. 81-89
Citations number
NO
Categorie Soggetti
Hematology
Journal title
Leukemia & lymphoma → ACNP
ISSN journal
10428194
Volume
11
Year of publication
1993
Supplement
1
Pages
81 - 89
Database
ISI
SICI code
1042-8194(1993)11:<81:WMCTRP>2.0.ZU;2-T
Abstract
The possible prognostic value of the position of the breakpoint within the M-BCR in patients with Ph 1 + CML is still being debated. We anal yzed the DNA rearrangements and the transcript types of 244 patients a nd tried to correlate the data obtained with prognostic features, defi ned according to Sokal's risk index, and with chronic phase and/or sur vival duration. The exact location of the breakpoint, either 5' or 3' to the Hind III restriction site within the M-BCR was identified. More over, the exact M-BCR subregion was also identified. As a whole, 150 p ts were rearranged in the 5' part and 94 in the 3' part of the M-BCR. No correlation was observed between the site of rearrangement on the o ne hand and the Sokal's prognostic index and survival, on the other. T ranscript analysis was performed in 130 patients; 59 carried an a2b2 a nd 69 an a,b3 pattern. Two patients carried both transcripts. Of the p atients rearranged in the 5' area, according to Southern blotting, 29. 2% showed an a,b3 transcript. Therefore, RT-PCR analysis allowed a bet ter definition of the breakpoint. However, also the type of transcript did not show any correlation either with risk categories or survival. No difference in response to therapy, either chemotherapy or alpha in terferon, was observed between 5' and 3' rearranged patients.