EXOTOXIN-INDUCED CONSUMPTIVE COAGULOPATHY IN ATLANTIC SALMON, SALMO-SALAR L - INHIBITORY EFFECTS OF EXOGENOUS ANTITHROMBIN AND ALPHA(2)-MACROGLOBULIN ON AEROMONAS-SALMONICIDA SERINE-PROTEASE
R. Salte et al., EXOTOXIN-INDUCED CONSUMPTIVE COAGULOPATHY IN ATLANTIC SALMON, SALMO-SALAR L - INHIBITORY EFFECTS OF EXOGENOUS ANTITHROMBIN AND ALPHA(2)-MACROGLOBULIN ON AEROMONAS-SALMONICIDA SERINE-PROTEASE, Journal of fish diseases, 16(5), 1993, pp. 425-435
Consumptive coagulopathy was induced within 4 h in Atlantic salmon, Sa
lmo salar L., by injecting purified serine protease from Aeromonas sal
monicida into the dorsal aorta. Pretreatment with a bolus intravascula
r injection of (human) antithrombin (AT) or (bovine) alpha2-macroglobu
lin (alpha2M) just prior to injection of the protease alleviated the i
n vivo pro-coagulant effects of the enzyme, but could not hinder the d
evelopment of consumptive coagulopathy. In fish receiving only saline
as pretreatment, the coagulopathy was evident even after 28 h, but the
fish were not overtly sick. The addition of the exogenous inhibitors
increased the fish's natural protection against the bacterial exotoxin
, suggesting that both AT and alpha2M are of importance for the outcom
e of the pathologic process. Results further indicate that while AT in
vivo was mainly directed against generated thrombin and activated coa
gulation factor X (FX(a)), alpha2M inhibited the protease directly.