A SIMPLE METHOD FOR THE PURIFICATION OF ORGANELLE DNA OF PLANTS

Authors
NATH P SANE AP BIJOLA V TRIVEDI PK ARORA J SANE PV
Citation
P. Nath et al., A SIMPLE METHOD FOR THE PURIFICATION OF ORGANELLE DNA OF PLANTS, Journal of Plant Biochemistry and Biotechnology, 2(2), 1993, pp. 117-120
Citations number
9
Categorie Soggetti
Biology,"Biothechnology & Applied Migrobiology
Journal title
Journal of Plant Biochemistry and Biotechnology → ACNP
ISSN journal
09717811
Volume
2
Issue
2
Year of publication
1993
Pages
117 - 120
Database
ISI
SICI code
0971-7811(1993)2:2<117:ASMFTP>2.0.ZU;2-C
Abstract
We report here a simple procedure for the purification of the organell e DNA. Mitochondrial DNA from Sorghum and the chloroplast DNA from Pop ulus and spinach were purified using this protocol. The method utilize s a quick centrifugation of the isolated organelle DNA through a two s tep CsCl density gradient for removal of small molecular weight nuclei c acids which pose a major problem for getting clean restriction patte rns. This method of purification can be adopted with any isolation pro cedure for organelle DNA.