CONSTRUCTION AND SCREENING OF BARLEY GENOMIC LIBRARY WITH A B(1)-HORDEIN GENE PROBE

Authors
TYAGI A SANTHA IM MEHTA SL
Citation
A. Tyagi et al., CONSTRUCTION AND SCREENING OF BARLEY GENOMIC LIBRARY WITH A B(1)-HORDEIN GENE PROBE, Journal of Plant Biochemistry and Biotechnology, 2(2), 1993, pp. 125-126
Citations number
10
Categorie Soggetti
Biology,"Biothechnology & Applied Migrobiology
Journal title
Journal of Plant Biochemistry and Biotechnology → ACNP
ISSN journal
09717811
Volume
2
Issue
2
Year of publication
1993
Pages
125 - 126
Database
ISI
SICI code
0971-7811(1993)2:2<125:CASOBG>2.0.ZU;2-1
Abstract
Barley genomic library from cv NP113 was made in a replacement vector EMBL-3. The titre was found to be 125 x 10(6) per mug of genomic DNA. The recombinants were screened using a B1-hordein-DNA probe. One clone contained a positively hybridizing 4 kb fragment.