A NEW METHOD FOR EX-VIVO WHOLE-BLOOD PERF USION OF ISOLATED ORGANS OFLARGE MAMMALS DEMONSTRATED IN PIG KIDNEYS

A new method for the ex vivo perfusion of organs from large mammals is described. Gas exchange and dialysis are carried out simultaneously w ith a low-flux polysulfon dialysis module. The dialysate (e.g. Tyrode solution) is aerated with a mixture of oxygen and carbon dioxide to en sure pas exchange with the blood. Dialysis is carried out in a closed thermostatically controlled system. Monitoring of ultrafiltration is m aintained by continuously weighing the blood reservoir and adjusting a n afferent and efferent blood pump. Initial results obtained with isol ated pig kidneys demonstrate the suitability of the new method for use as a model for the replacement of animal experiments. Theoretically, clinical application in the area of in vivo regional organ perfusion m ay also be possible.