COVALENT ATTACHMENT OF HYBRIDIZABLE OLIGONUCLEOTIDES TO GLASS SUPPORTS

A simple, rapid, and efficient method for the covalent binding of olig onucleotides to solid glass supports was developed. Glass slides were derivatized with aminophenyl or aminopropyl silanes and 5'-succinylate d target oligonucleotides were attached by carbodiimide-mediated coupl ing. Approximately 40 to 50% of the applied target oligonucleotides co valently bound to the derivatized glass. Hybridizations with radioacti vely labeled oligonucleotide probes showed that up to 90% of the attac hed oligonucleotides were available for hybridization. This system can conveniently be applied for studies on hybridization and detection of nucleic acids. (C) 1997 Academic Press.