IMPROVED METHOD FOR THE RECOVERY OF ASCARUS-SUUM LARVAE FROM PIG INTESTINAL-MUCOSA

In the course of a comprehensive study on the population biology of As caris suum, it became necessary to determine quantitatively the migrat ion of the larvae (L(2)) through the pig intestine. Because no satisfa ctory methods for recovering these larvae from the intestinal mucosa w ere apparently available in the literature, we undertook the developme nt of such a procedure. Direct Baermannization of the intestine proved inadequate, so a series of studies was undertaken to find a method to solubilize the mucosa and free the larvae. Both pepsin digestion and mucus reduction with N-acetyl cysteine were evaluated. The highest rec overy rates were obtained with short-term pepsin digestion (45-90 min) , followed by a specific settling and washing procedure. A third alter native, migration of larvae out of agar-gel, was also tested. Although the recovery efficiency was low (10% of pepsin digestion), the larvae recovered were clean, with good motility. The agar-gel procedure coul d be useful when L(2) larvae are needed for other purposes, such as an tigen preparation or cultivation.