T. Hollis et al., Crystallization and preliminary X-ray analysis of a chitinase from the fungal pathogen Coccidioides immitis, ACT CRYST D, 54, 1998, pp. 1412-1413
Chitinase is necessary for fungal growth and cell division and, therefore,
is an ideal target for the design of inhibitors which may act as antifungal
agents. A chitinase from the fungal pathogen Coccidioides immitis has been
expressed as a fusion protein with gluathione-S-transferase (GST), which a
ids in purification. After cleavage from GST, chitinase was crystallized fr
om 30% PEG 4000 in 0.1 M sodium acetate pH 4.6. The crystals have a tetrago
nal crystal lattice and belong to space group P4(1)2(1)2 or P4(3)2(1)2 and
diffract to 2.2 Angstrom resolution. The unit-cell parameters are a = b = 9
1.2, c = 95.4 Angstrom; there is only one chitinase molecule in the asymmet
ric unit.