Crystallization and preliminary X-ray diffraction study of lactonohydrolase from Fusarium oxysporum

The lactonohydrolase from the fungus Fusarium oxysporum AKU 3702 was crysta llized by the vapour-diffusion procedure in the presence of polyethylene gl ycol 4000 as a precipitant. The crystals belong to the monoclinic space gro up P2(1) with unit-cell parameters a = 156, b = 100, c = 94.1 Angstrom, bet a = 91.7 degrees. Assuming that there are two lactonohydrolase-dimer molecu les in the asymmetric unit, the crystal volume per unit molecular mass. V-m , is calculated to be 2.94 Angstrom(3) Da(-1).