G. Nilsson et al., Reverse transcriptase polymerase chain reaction on fine needle aspirates for rapid detection of translocations in synovial sarcoma, ACT CYTOL, 42(6), 1998, pp. 1317-1324
Citations number
20
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
OBJECTIVE: To evaluate the utilization of fine needle aspiration (FNA) biop
sy to obtain material for revers-transcriptase polymerase chain reaction (R
T-PCR) in the detection of the t(X;18)(p11.2;q11.2) translocation in synovi
al sarcomas.
STUDY DESIGN: We applied RT-PCR to detection of synovial sarcoma fusion gen
e transcripts on fine needle aspirates. Five clinical samples were first an
alyzed: one was a tumor previously diagnosed as malignant hemangiopericytom
a, one was a poorly defined tumor, and three were suspected synovial sarcom
as. FNA material was transferred directly to the RT-PCR reaction tube witho
ut RNA extraction.
RESULTS: The t(X;18) translocation could be detected on the limited amount
of material that FNA provides. In each of the cases studied the representiv
ity of the tumor samples was confirmed microscopically.
CONCLUSION: Our protocol permits analysis directly on representative sample
s without extraction of RNA. The results imply that RT-PCR offers reliable
detection of sarcoma fusion gene transcripts on fine needle aspirates. The
procedure, apart from being applicable to outpatients, is rapid and sensiti
ve.