Reverse transcriptase polymerase chain reaction on fine needle aspirates for rapid detection of translocations in synovial sarcoma

Citation
G. Nilsson et al., Reverse transcriptase polymerase chain reaction on fine needle aspirates for rapid detection of translocations in synovial sarcoma, ACT CYTOL, 42(6), 1998, pp. 1317-1324
Citations number
20
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Journal title
ACTA CYTOLOGICA
ISSN journal
00015547 → ACNP
Volume
42
Issue
6
Year of publication
1998
Pages
1317 - 1324
Database
ISI
SICI code
0001-5547(199811/12)42:6<1317:RTPCRO>2.0.ZU;2-4
Abstract
OBJECTIVE: To evaluate the utilization of fine needle aspiration (FNA) biop sy to obtain material for revers-transcriptase polymerase chain reaction (R T-PCR) in the detection of the t(X;18)(p11.2;q11.2) translocation in synovi al sarcomas. STUDY DESIGN: We applied RT-PCR to detection of synovial sarcoma fusion gen e transcripts on fine needle aspirates. Five clinical samples were first an alyzed: one was a tumor previously diagnosed as malignant hemangiopericytom a, one was a poorly defined tumor, and three were suspected synovial sarcom as. FNA material was transferred directly to the RT-PCR reaction tube witho ut RNA extraction. RESULTS: The t(X;18) translocation could be detected on the limited amount of material that FNA provides. In each of the cases studied the representiv ity of the tumor samples was confirmed microscopically. CONCLUSION: Our protocol permits analysis directly on representative sample s without extraction of RNA. The results imply that RT-PCR offers reliable detection of sarcoma fusion gene transcripts on fine needle aspirates. The procedure, apart from being applicable to outpatients, is rapid and sensiti ve.