Z. Svobodova et al., The first findings of the differences in complete blood count between diploid and triploid tench, Tinca tinca L., ACT VET B, 67(4), 1998, pp. 243
The aim of this work was to evaluate differences in complete blood count of
diploid and triploid tench and concentration of total protein in blood pla
sma of these fish. Haematological examination of 78 specimens of three-year
-old tench, consisting of 21 triploids and their 57 diploid siblings was pe
rformed in July 1996. Analysis of variance test confirmed a significantly l
ower erythrocyte count in triploids (P < 0.01), as well as a significantly
lower haemoglobin content (P < 0.05), significantly higher medium corpuscul
ar volume, higher medium corpuscular haemoglobin and lower medium corpuscul
ar haemoglobin concentration (all at P < 0.01) compared to diploids. The ha
ematocrit value of triploids was insignificantly lower compared to diploids
. The differences of erythrocyte nuclear area, perimeter, average size, maj
or axis and aspect ratio between diploids and triploids were found signific
ant at P < 0.01 and the effect of ploidy level on these dimensions was cons
idered highly significant (P < 0.0001). No effect of ploidy level was prove
n for erythrocyte nuclear minor axis. The leukocyte count of triploids was
insignificantly lesser compared to diploids. Triploid tench were found of s
ignificantly lower concentration of total protein in blood plasma compared
to diploids (P < 0.01). Triploid tench could be hypothesized to have a lowe
r oxygenation capacity of blood, as well as a lower non-specific immunity l
evel compared to diploids. Insignificantly higher erythrocyte count, haemog
lobin content, haematocrit value, and significantly higher concentration of
total protein in blood plasma (P < 0.01) was reported for diploid females
compared to males.