Ma. Ossevoort et al., Use of in vitro correlates for selection of candidate immunosuppressive antibodies prior to a primate transplant model, ALTEX-AL TI, 15, 1998, pp. 23-26
The selection of possible candidate immunosuppressive antibodies to prevent
graft rejection is performed in vitro. Additionally, due to the species sp
ecificity of these monoclonal antibodies (MABs), pre-clinical studies in no
n-human primates are necessary. If a positive correlation between the in vi
tro and in vivo findings would exist, these tests can act as a prescreening
before new reagents are tested in vivo. The correlation of the in vitro an
d in vivo efficacy of an anti-T-lymphocyte globulin (ATG) and an anti-CD80
MAB is evaluated in a rhesus monkey skin transplant model. The results show
that lymphocytotoxic titers (NIH-test) do not predict the outcome of in vi
vo effectiveness of ATG in rhesus monkeys. Additionally, no evidence of tol
erance to a skin allograft could be shown to correlate with inhibition of a
secondary mixed lymphocyte culture (MLC) by anti-CD80 and cyclosporin A (C
sA). Thus, these in vitro assay used can not predict the in vivo efficacy o
f new immunosuppressive antibodies.