Optimal sample and plot size for inventory of field and ground layer vegetation in a mature Myrtillus-type boreal spruce forest

Field and ground layer vegetation in a mature Norway spruce (Picea abies L. ) forest of Myrtillus-type in Kuorevesi, southern Finland, was sampled on 3 1 plots of sizes 0.5, 1 and 2 m(2) to find optimal balance among sampling e ffort, number of observed species, and accuracy of mean species-cover estim ates. Rarefaction and bootstrapping were used in the data analysis. Accordi ng to the results, the addition of species is slow yet continuous after the first few plots. The expected number of species in samples taken with the different plot sizes decreases in the following order: 2 > 1 > 0.5 m(2). Re sults implied that plant species have different optimum plot sizes with res pect to the accuracy of the mean cover estimates. However, representative r egistering of species present needs a larger sample and plot size than a re latively accurate estimation of the mean cover of the individual species. C onsequently, representative registering of species sets the minimum limits for the number and size of the sample plots.