Jy. Honda et al., Use of the internal transcribed spacer (ITS-1) region to infer Orius (Hemiptera : Anthocoridae) species phylogeny, APPL ENT ZO, 33(4), 1998, pp. 567-571
Partial 18S, 5.8S and the intervening internal transcribed spacer region 1
(ITS-1) of nuclear ribosomal DNA from representatives of 10 species of the
genus Orius and one outgroup species (Wollastoniella rotunda Yasunaga et Mi
yamoto) were analyzed by PCR and DNA sequencing. Within the taxa analyzed,
the length of the ITS-1 region varied from 230-663 bp. Phylogenetic trees g
enerated from informative sites within the sequences were highly resolved i
n the proximal regions of the branches, but more ambiguous deeper within th
e tree. Orius species relationships showed some agreement with anecdotal mo
rphological observations and this study represents a first attempt in estab
lishing Orius species relationships.