Three new continuous fish cell lines of SPH, SPS and RSBF derived from seaperch (Lateolabrax japonicus) and red sea bream (Pagrosomus major).

The continuous fish cell lines SPH and SPS were derived from heart and sple en tissues of sea perch Lateolabrax japonicus in 1993, and the cell line RS BF from fin tissue of red sea bream Pagrosomus major in 1994. They were sub cultured for 106, 102 and 96 times respectively in the medium Eagle's MEM s upplemented with 10% FBS. The growth temperature range was 15 to 30 degrees C for all of the cell lines with the optimum growth at 25 degrees C for SP H and SPS and at 30 degrees C for RSBF. The distribution of chromosome numb er was 30 to 96 for SPH at passage 70, 36 to 86 for SPS at passage 65 and 1 0 to 62 for RSBF at passage 62, Nevertheless, the modal number of the chrom osomes of all cell lines was 48. All of the cell lines were susceptible to fish birnavirus IPNV, though the titers were relatively low. (C) 1998 Publi shed by Elsevier Science B.V. All rights reserved.