Molecular systematics of subtribe Banksiinae (Banksia and Dryandra; proteaceae) based on cpDNA and nrDNA sequence data: Implications for taxonomy andbiogeography
Ar. Mast, Molecular systematics of subtribe Banksiinae (Banksia and Dryandra; proteaceae) based on cpDNA and nrDNA sequence data: Implications for taxonomy andbiogeography, AUST SYST B, 11(3-4), 1998, pp. 321-342
Despite considerable research interest in the subtribe Banksiinae (Banksia
L.f. and Dryandra R.Br.), no strongly supported phylogenetic hypothesis for
the relationship between the genera exists, nor have molecular characters
been sampled for phylogenetic reconstruction at any level. In this study, D
NA sequence characters were sampled from chloroplast DNA (cpDNA; the trnL i
ntron, the trnL 31 exon, and the spacer between the trnL 3' exon and trnF)
and nuclear ribosomal DNA (nrDNA; both internal transcribed spacers) of 18
species of Banksia and five of Dryandra, with six outgroup taxa from the su
bfamily Grevilleoideae. The molecular characters provided the opportunity t
o code taxa outside of Banksia for cladistic comparison with the genus-an o
pportunity not previously provided by morphological characters. Cladistic a
nalyses, using parsimony, explored the effects of various weightings of tra
nsition to transversion events and base substitution to insertion and delet
ion events to determine which relationships in the cladograms were robust.
The trnL/trnF and ITS characters strongly supported a paraphyletic Banksia
with respect to a monophyletic Dryandra. The molecular results supported a
single root for Thiele and Ladiges' (1996) unrooted morphological cladogram
along the branch between the Isotylis to B. fuscolutea clade and the Grand
es to B. tricuspis clade. George's (1981) subgenus Banksia and section Bank
sia appeared dramatically non-monophyletic, The distribution of eastern tax
a at derived positions on the molecular cladograms suggested considerable c
ladogenesis in the the genus prior to the formation of the Nullarbor Plain
during the Tertiary.