Root induction in Simmondsia chinensis (Link) Schneid. using Agrobacteriumrhizogenes.

Root induction on in vitro Simmondsia chinensis shoots was improved after 6 0 days cocultivation with Agrobacterium rhizogenes, with or without 490 mu M IBA simultaneously added. The three strains used (A4, 1601 and 9402) incr eased the rooting percentage to 80, 73.4 and 73.3%, respectively. IBA produ ced calli at the base of the stem in similar percentages than 9402 or 1601 strains, but calli was notably reduced when A4 strain or combined treatment s (Agrobacterium and IBA) were used. Cefotaxime improved rooting percentage respect to the controls, but this response was significantly increased bet ween 86 to 100% when A4 strain was used, solely or combined with IBA. Root number and root length were significantly enhanced at 523.6 mu M cefotaxime respect to uninfected plants. Combined bacteria-IBA-cefotaxime treatments significantly augmented all studied parameters. Negative opine detection di d not let us confirm that the roots formed under the Agrobacterium influenc e were really transgenic.