Ac. Gazdag et al., Effect of extracellular palmitate on 2-deoxy-D-glucose uptake in muscle from ad libitum fed and calorie restricted rats, BIOC BIOP R, 252(3), 1998, pp. 733-737
Citations number
40
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
We studied the effect of a high physiologic concentration of palmitate (1mM
) on in vitro 2-deoxy-D-glucose (2DG) uptake by flexor digitorum brevis (FD
B) muscle from ad libitum fed rats (AL) and rats fed 60% of ad libitum inta
ke (CR) for 20 days. CR did not alter muscle 2DG uptake in the absence of i
nsulin, but relative to AL, CR significantly (p<0.01) increased 2DG uptake
in the presence of 20,000 mu U/ml insulin. This effect of CR persisted in t
he presence of 1mM palmitate. The presence of 1mM palmitate significantly (
p<0.01) impaired 2DG; glucose uptake, both in the presence and absence of i
nsulin, to the same extent in AL and CR muscle, despite an 18% decrease in
FABPpm expression with CR. Thus, although CR profoundly affects insulin-med
iated muscle glucose uptake, it does not alter the ability of extracellular
fatty acid to modulate glucose utilization by skeletal muscle. (C) 1998 Ac
ademic Press.