Characterization of the human proline/arginine-rich end leucine-rich repeat protein (PRELP) gene promoter and identification of a repressor element

The 5'-flanking region of the human proline/arginine-rich end leucine-rich repeat protein (PRELP) gene has been characterized for both promoter and re pressor activity by using a variety of reporter gene constructs and transie nt transfection into chondrocytes or fibroblasts. The human PRELP gene lack s a TATA box, and in its absence a Sp1-binding site residing 29 bp upstream of the transcription start site is essential for initiating gene expressio n. In contrast, an Ets-binding site residing 497 bp up-stream of the transc ription start site can lead to the repression of gene expression. The analy sis of nuclear proteins by gel retardation studies with the repressor eleme nt identified a common protein, presumably an Ets family member, present in neonatal chondrocytes and skin fibroblasts that do not express the PRELP g ene. The factor was not detected in nuclear protein preparations from adult chondrocytes in which the PRELP gene is expressed.