REAL-TIME ANALYSIS OF ANTIBODY-BINDING INTERACTIONS WITH IMMOBILIZED ESCHERICHIA-COLI 0157-H7 CELLS USING THE BIACORE

The kinetic properties of antibody-antigen reaction and other interact ing macromolecules can be analyzed in realtime using the surface plasm on resonance biosensor (BIAcore). The interactions of an antibody agai nst Escherischia coli O157:H7 were studied using immobilized whole cel ls. The bacterial sensor surface was evaluated with anti-E. coli in a continuous flow system. Regeneration of the sensor surface with guanid ine-HCl was more effective than with phosphoric acid and resulted in b etter binding reproducibility. The determined kinetic values, associat ion and dissociation rate constants, can be used in the development of rapid immune-techniques. This study also provides the basis to evalua te real-time interactions of macromolecules with immobilized cells.