Z. Antal et al., TRANSFORMATION OF A MYCOPARASITIC TRICHODERMA-HARZIANUM STRAIN WITH THE ARGB GENE OF ASPERGILLUS-NIDULANS, Biotechnology techniques, 11(3), 1997, pp. 205-208
A transformation system was developed for the mycoparasitic filamentou
s fungus Trichoderma harzianum, based upon complementation of auxotrop
hic mutants. Prototrophic transformants were obtained using plasmids p
Sa123 and pAN5-41B, carrying the Aspergillus nidulans argB gene, and b
oth the Aspergillus nidulans argB and Escherichia coli lacZ genes, res
pectively.