A novel catechol oxidase enzyme electrode for the specific determination of catechol

An enzyme electrode for the specific determination of catechol was develope d by using catechol oxidase (EC 1.10.3.1) from eggplant (Solanum melangena L.) in combination with a dissolved oxygen probe. Optimization studies of t he prepared catechol oxidase enzyme electrode established a phosphate buffe r 50 mM at pH 7.0 and 35 degrees C to provide the optimum conditions for af firmative electrode response. The enzyme electrode response depended linear ly on a catechol concentration range of 5.10(-7)-30.10(-5) M with a respons e time of 25 sec and substrate specificity of the catechol oxidase electrod e of 100%. The biosensor retained its enzyme activity for at least 70 days.