Structural study on a sulfated polysaccharide-peptidoglycan complex produced by Arthrobacter sp.

The structure of a sulfated polysaccharide-peptidoglycan complex (SP-PG) pr oduced by Arthrobacter sp. was analyzed by NMR spectroscopy. In addition, o ligosaccharide fragments of the SP-PG-L obtained by HF degradation were ana lyzed by NMR spectroscopy. These findings indicated that the sulfated polys accharide (SP) contains a repeating unit composed of two galactofuranosides and a glucopyranoside. The main chain of the trisaccharide is [-->6)beta-D -Galf(1-->6)-beta-D-Galf(1-->](n), with beta-D-Glcp linked to one of the Ga lfs through a (1-->2) linkage. The sulfated positions of the trisaccharide were identified as C-3 and C-5 of the beta-glucosylated Galf residues, and C-2 or C-3 of the other Galf residue.