PCR method for detecting recombinant baculoviruses with the insertion of alarge gene

A PCR strategy was developed using primers specific to an infectious bursal disease virus (IBDV) gene as well as primers flanking the polyhedrin regio n of baculovirus to verify the presence of IBDV gene in the recombinant bac ulovirus and confirm the absence of wild-type baculovirus contamination. Th is method can be applied to detect the presence of large genes in the recom binant baculovirus with greater sensitivity and avoid the need of modifying the typical PCR procedure provided by the manufacturer.