New procedure for the determination of nisin in milk

Authors
Rossano, R Del Fiore, A D'Elia, A Pesole, G Parente, E Riccio, P
Citation
R. Rossano et al., New procedure for the determination of nisin in milk, BIOTECH TEC, 12(10), 1998, pp. 783-786
Citations number
14
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOTECHNOLOGY TECHNIQUES
ISSN journal
0951208X → ACNP
Volume
12
Issue
10
Year of publication
1998
Pages
783 - 786
Database
ISI
SICI code
0951-208X(199810)12:10<783:NPFTDO>2.0.ZU;2-X
Abstract
A new procedure is described for the assay of nisin in milk by using capill ary zonal electrophoresis. Since nisin assay in milk and in other matrices is hampered by its adsorption to both the protein and lipid milk fractions, a simple extraction procedure was devised to remove caseins and lipids, wh ich interfere with nisin detection. An excellent linear response to nisin c oncentration was observed in the range from 10 to 100 mu g/ml with a mean c oefficient of variation of 3.8%.