The effects of maternal intake and gestational age on materno-fetal transport of vitamin C in the guinea-pig

The effects of maternal intake and gestational age on materno-fetal transpo rt of vitamin C were investigated in female Dunkin-Hartley guinea-pigs. Twe nty-four time-mated dams were fed on either a moderate-vitamin C (group A) or a high-vitamin C (group B) diet, throughout pregnancy. At days 49, 63 an d 66 of gestation, and at term, three animals from each group were killed a nd fetuses removed by hysterectomy. Plasma, liver, kidney, lungs, heart, pl acenta and amniotic fluid were collected from dams and fetuses and subseque ntly analysed for vitamin C. Fetal plasma and amniotic fluid concentrations showed negative associations with gestational age for both dietary groups, independent of an effect of dam (P < 0.001). A similar, though not signifi cant, effect of gestational age was observed on placental vitamin C and gro up A fetal tissue concentrations. Fetal plasma, placental and amniotic flui d vitamin C concentrations were significantly higher in group B than group A (P < 0.001), suggesting the influence of maternal diet. Although the leve l of maternal vitamin C intake can influence the circulating concentration in the fetus, there is a decrease associated with increasing; gestational a ge, independent of the maternal diet. The gestational age-dependent change in the fetal accumulation of vitamin C may reflect changes in the rate of p lacental transfer.