Differences in the microbial communities associated with the roots of different cultivars of canola and wheat

Citation
Sd. Siciliano et al., Differences in the microbial communities associated with the roots of different cultivars of canola and wheat, CAN J MICRO, 44(9), 1998, pp. 844-851
Citations number
34
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
CANADIAN JOURNAL OF MICROBIOLOGY
ISSN journal
00084166 → ACNP
Volume
44
Issue
9
Year of publication
1998
Pages
844 - 851
Database
ISI
SICI code
0008-4166(199809)44:9<844:DITMCA>2.0.ZU;2-X
Abstract
Plant characteristics are known to alter endophytic and rhizosphere microbi al communities; however, the effect of crop breeding programs on the microb ial endophytic and rhizosphere communities is not clear. The purpose of thi s study was to determine if root-associated microbial communities differed between three cultivars of canola (Brassica spp.) and wheat (Triticum spp.) . Biolog(TM) analysis was used to characterize the microbial communities as sociated with the root interior and rhizosphere: soil of field grown canola (Parkland, Excel, and Quest cultivars) as well as wheat (PI 167549, Red Fi fe,and CDC Teal cultivars). Fatty acid methyl ester (FAME) profiles of root s and rhizosphere soil of the cultivars were also compared. These crop cult ivars represent a continuum from older to the most recent crop cultivars, w ith Quest being a transgenic canola variety tolerant of the herbicide glyph osate. To the best of our knowledge, Quest is not directly related to Parkl and or Excel. The endophytic community of Quest used the Biolog(TM) polymer , carbohydrate, amino acid, and miscellaneous functional guilds at a slower rate than the endophytic community of Excel or Parkland, Furthermore, ther e were lower levels of the microbial FAMEs, 18:0, 18:3 w6c (6,9,12), 16:0 2 OH, and 15:0 2OH in the roots of Quest compared with Excel or Parkland. In contrast, there were no differences in the utilization rate of Biolog(TM) f unctional guilds or the microbial FAMEs in the roots of the three wheat cul tivars studied. The correlation between the ability of endophytic and rhizo sphere communities to utilize Biolog(TM) substrates was lower in Quest and CDC Teal compared with earlier crop cultivars. Our results indicate that en dophytic and rhizosphere microbial communities of the transgenic cultivar Q uest were different from nontransgenic cultivars grown at the same field si te.