Room temperature induced apoptosis of Jurkat cells sensitive to both caspase-1 and caspase-3 inhibitors

We recently reported that HL-60 cells underwent apoptosis when exposed to r oom temperature (RT) (21 degrees C), RT-induced apoptosis of HL-60 cells is inhibited by the caspase-1 inhibitor (YVAD-CMK), but not by the caspase-3 inhibitor (DEVD-CHO). In this study, we studied RT-induced apoptosis in 15 human cell lines of hematopoietic lineage and found that the Jurkat cell li ne also responded to RT by a different apoptotic process. RT-induced apopto sis of Jurkat cells was attenuated by YVAD-CMK as well as DEVD-CHO. Increas ed caspase activity on DEVD-AMC, which was inhibited by both YVAD-CMK and D EVD-CHO added to the cell culture, was also detected. The involvement of ca spase-3 itself, however, was not recognized by Western blot analysis. In co ntrast, the processing of caspase-3 was observed in the apoptotic HL-60 cel ls. These data implicate the presence of the redundant processes of apoptos is induced by RT treatment. (C) 1998 Elsevier Science Ireland Ltd. All righ ts reserved.