Putrescine and chlorpheniramine inhibit Ehrlich ascites tumor cell plasma membrane ferricyanide reductase activity

The presence of putrescine or chlorpheniramine in the incubation medium of Ehrlich ascites tumor cells starved for 1 h significantly inhibits the rate of ferricyanide reduction by their plasma membrane redox system. Freshly h arvested cells, without depletion of their intracellular pools of polyamine s, and cells preincubated under conditions arranged to increase ornithine d ecarboxylase activity also reduced externally added ferricyanide at a lower rate than those cells starved for 1 h. All these data seems to indicate th at the presence of putrescine is enough to significantly inhibit Ehrlich ce ll plasma membrane redox system activity. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.