Time-resolved UV-Vis spectroelectrochemical studies of the conformational rearrangement in the electron transfer of cytochrome c

The present work used mainly electrochemical in situ time-resolved W-Vis su btractive reflectance spectroscopy to study the electron transfer process o f cytochrome c in the presence of promoter(the equal molal mixture of guani ne and cytosine). Three new absorption bands, 420 nm, 450 nm and 500 nm, ar e observed in the spectra with different time resolutions, as well as two b ipolar absorption bands 250 nm and 300 nm. All the results show that the el ectron transfer of cytochrome c is accompanied by significant conformationa l rearrangement.