THE NITRATE REDUCTASE GENE ISOLATED FROM DNA OF CULTURED SPINACH CELLS

Authors
TAMURA N TAKAHASHI H TAKEBA G SATOI T NAKAGAWA H
Citation
N. Tamura et al., THE NITRATE REDUCTASE GENE ISOLATED FROM DNA OF CULTURED SPINACH CELLS, Biochimica et biophysica acta. Protein structure and molecular enzymology, 1338(2), 1997, pp. 151-155
Citations number
15
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta. Protein structure and molecular enzymology → ACNP
ISSN journal
01674838
Volume
1338
Issue
2
Year of publication
1997
Pages
151 - 155
Database
ISI
SICI code
0167-4838(1997)1338:2<151:TNRGIF>2.0.ZU;2-W
Abstract
The gene encoding nitrate reductase was cloned from the DNA isolated f rom cultured spinach (Spinacia oleracea cv. Hoyo) cells and sequenced. The clone contains 7612 nucleotides of the gene which consists of fou r exons interrupted by three introns. The transcription start site was determined by primer extension analysis and located 193 bp upstream o f the ATG translation initiation codon. The 5'-flanking region contain s a TATA box and CAAT box. (C) 1997 Elsevier Science B.V.