Evidence for a frizzled-mediated wnt pathway required for zebrafish dorsalmesoderm formation

Citation
A. Nasevicius et al., Evidence for a frizzled-mediated wnt pathway required for zebrafish dorsalmesoderm formation, DEVELOPMENT, 125(21), 1998, pp. 4283-4292
Citations number
44
Categorie Soggetti
Cell & Developmental Biology
Journal title
DEVELOPMENT
ISSN journal
09501991 → ACNP
Volume
125
Issue
21
Year of publication
1998
Pages
4283 - 4292
Database
ISI
SICI code
0950-1991(199811)125:21<4283:EFAFWP>2.0.ZU;2-4
Abstract
We have used zebrafish as a model system for the study of vertebrate dorsov entral patterning. We isolated a maternally expressed and dorsal organizer localized member of the frizzled family of wnt receptors. Wild-type and dom inant, loss-of-function molecules in misexpression studies demonstrate friz zled function is necessary and sufficient for dorsal mesoderm specification . frizzled activity is antagonized by the action of GSK-3, and we show GSK- 3 is also required for zebrafish dorsal mesoderm formation. frizzled cooper atively interacts with the maternally encoded zebrafish wnt8 protein in dor sal mesodermal fate determination. This frizzled-mediated wnt pathway for d orsal mesoderm specification provides the first evidence for the requiremen t of a wnt-like signal in vertebrate axis determination.