Pure fraction PRF3, hydroxyproline - rich glycoprotein, was isolated f
rom soybean cell wall material (dietary fibre) through sodium chlorite
- acetic acid (NaClO2 - HOAc) extraction and chromatographic column p
urification. The results of intensive structural characterization show
ed that PRF3 contained beta(1-->4) linked galactan backbone which was
branched at position 3 -C with alpha(1-->3) linked oligoarabinan as si
de chains, and the polypeptide was glycosidically linked through hydro
xyprolinyl arabinosides and serinyl galactosides.