Assessment of iodine deficiency and monitoring of iodine supplementation pr
ograms demands rapid simple and cost-effective methods for the determinatio
n of urinary iodide concentrations. We propose a rapid test based on the io
dide-catalyzed oxidation of 3,3 ',5,5'-tetramethylbenzidine by peracetic ac
id/H2O2 to yield colored products. The color of the chemical reaction is co
mpared with color categories of a pictogram corresponding to three ranges (
<10, 10-30, and much greater than 30 mu g/100 mL) of iodide concentrations.
The test is very easy to perform and does not require any instrumentation
or apparatus. Sample preparation is simple and consists in the removal of i
nterfering substances by disposable columns, 65 x 10.5 mm, packed with spec
ifically prepared activated charcoal. For comparison with a reference metho
d for measuring urinary iodide (HPLC), we determined the iodide concentrati
ons of 370 random (untimed) urine samples from consecutive patients by both
HPLC and the rapid test.
The results obtained by both methods are in close agreement with respect to
classification of the samples according to the above three ranges, with a
maximum difference of <5% for each range. This rapid test is therefore very
well suited to epidemiological surveys of iodine deficiency especially in
developing countries.