F. Rudert et al., A phage-based system to select multiple protein-protein interactions simultaneously from combinatorial libraries, FEBS LETTER, 440(1-2), 1998, pp. 135-140
Selectively infective phage (SIP) can be used to identify protein-protein i
nteractions. SIP was modified to facilitate the simultaneous selection of i
nteracting protein pairs from large combinatorial libraries. An interferenc
e-resistant phage mas constructed which non-covalently, but stably links th
e genetic information of an interacting pair, encoded separately on phage a
nd phagemid vectors, by co-packaging into hetero-polyphages. In a model sys
tem, the interaction between a SIP-selected peptide and the intracellular d
omain of the p75 neurotrophin receptor was detected in the presence of a 10
(4)-fold excess of a non-interacting control pair (jun leucine zipper and p
75 intracellular domain) via SIP hetero-polyphage transductants. To minimiz
e the redundancy of transductants and to minimize possible ligand exchange
generated in a solution-based SIP screening, a filter-based in situ infecti
vity screening aas developed, The combination of the above techniques may p
rovide a powerful system for rapid screening of very large sequence spaces.
(C) 1998 Federation of European Biochemical Societies.