Fj. Alarcon et al., Characterization and functional properties of digestive proteases in two sparids; gilthead seabream (Sparus aurata) and common dentex (Dentex dentex), FISH PHYS B, 19(3), 1998, pp. 257-267
Digestive proteases present in two sparids, seabream (Sparus aurata) and co
mmon dentex (Dentex dentex), have been characterized using both biochemical
and electrophoretic techniques. Although optimum pH and temperature for ma
ximum activity of both acid and alkaline proteases were similar in the two
species, important differences in total activity, as well as in thermal and
pH stability were found. Specific inhibitors and SDS-PAGE zymograms were u
sed to clarify such differences. Evidences support the existence of a more
active and complex protease set in dentex. Results are discussed from the p
erspective of their application to the formulation of feeds for each specie
s.