Human GBF1 is a ubiquitously expressed gene of the Sec7 domain family mapping to 10q24

We present the cDNA sequence of human GBF1 along with data on expression pa ttern and genomic attributes. The deduced polypeptide encodes a putative gu anine nucleotide exchange factor of 206.5 kDa, containing a centrally posit ioned Sec7 domain and a proline-rich region at the extreme C terminus. Its mRNA transcripts are found in 17 tissues and cell lines, likely suggesting a housekeeping role for GBF1p. The gene maps to 10q24, 2.33 cR from D10S540 . It is fully contained within YAC 822C1 and covers at most 450 kb. Its Sec 7 domain-encoding region harbors four introns. (C) 1998 Academic Press.