Circulating concentrations of placenta protein 14 during the natural menstrual cycle in women significantly reflect endometrial receptivity to implantation and pregnancy during successive assisted reproduction cycles

Placenta protein 14 (PP14), which is the most abundant product of the secre tory endometriun, has been proposed as the best biochemical marker of endom etrial function in women. In this study, 19 normogonadotrophic women of inf ertile couples were monitored with serial measurements of concentrations of PP14, gonadotrophins and sex steroids and ultrasound scanning of endometri al thickness throughout three consecutive cycles. The first two of these we re natural, unstimulated cycles (cycles 1 and 2), while ovarian stimulation with clomiphene and human menopausal gonadotrophin combined with assisted reproduction (intrauterine insemination in four cases and in-vitro fertiliz ation in 15) was performed in the third cycle (cycle 3), A newly developed enzyme-linked immunosorbent assay was used to measure serum PP14 concentrat ions. In cycle 3, seven women became pregnant (group A) and 12 did not (gro up B), Circulating concentrations of PP14 were significantly lower in group A than in group B throughout all three cycles and in all cycle phases with exception of the late luteal phase of cycle 3, during which PP14 concentra tions in group A were significantly higher than in group B. Statistical ana lyses showed no significant correlations between serum concentrations of PP 14 and follicle stimulating hormone, luteinizing hormone and progesterone, and endometrial thickness. By contrast, serum oestradiol concentrations dur ing the pre-ovulatory phase were significantly correlated with PP14 concent rations during the mid-luteal phase of the cycle. It is concluded that circ ulating PP14 is a most reliable biochemical marker of endometrial function in women and that relatively low concentrations in serum during the natural , unstimulated cycle are significantly correlated to implantation and pregn ancy during successive assisted reproduction cycles. Measurement of PP14 in serum may thus be useful as a method of screening endometrial function in women, before commencing troublesome and costly treatment for infertility, However, further studies in a much larger number of women are needed to con firm this observation and to elucidate the as yet undefined physiological f unctions of PP14 in women.