Detailed deletion mapping on chromosome 1p32-p36 in human colorectal cancer: Identification of three distinct regions of common allelic loss

Recent studies have suggested the existence of one or several tumor-suppres sor genes on chromosome arm 1p in colorectal tumors. To determine the local ization of the putative tumor suppressor genes, we performed LOH analysis i n 1p in colorectal tumors. A total of 48 paired normal and tumor DNAs of 46 colorectal tumor patients and 21 microsatellite markers on 1p32.1-p36.3 we re used for PCR-LOH analysis. Three commonly deleted regions were found: i) 1p36.3 (10-cM); ii) 1p35.1-p36.3 (2-cM); and iii) 1p34.2-p35 (1-cM). These regions overlapped with those reported in several types of tumor. No signi ficant associations were found between LOH and clinicopathologic features. The regions identified in the present study could harbor tumor suppressor g enes that would also be associated with several types of human cancer.