Determination of apramycin in swine kidney tissue by liquid chromatographywith fluorescence detection

A liquid chromatographic method for determining apramycin in swine kidney t issue is described. Apramycin is extracted from tissue with basic methanol and purified by ion-pair extraction. By using an automated derivatization a nd injection procedure, the purified extract is derivatized with o-phthalde hyde, separated on a C-18 column, and detected with a fluorescence detector . For fortified kidney samples, between-run coefficients of variation range d from 4.8 to 7.1% at 1.00 ppm and from 9.6 to 14.3% at 0.50 ppm. Recoverie s ranged from 76 to 86%. Standard curves were linear over the range 10-100 ng/mL.